Lakes developed on progressively younger end moraines of the Klutlan Glacier were initially assumed to have originated shortly after moraine emplacement and to have persisted to the present. Limnological differences between lakes on old vs young moraines were thought to result from limnological maturation within the lakes and ponds themselves and in response to the development of soils and vegetation on moraine surfaces. This study represents a paleolimnological test of this hypothesis. If true, the first-formed sediments of lakes on old moraines should be comparable to sediments presently forming in lakes on young moraines. Geochemical and paleontological studies of surface sediment to a series of lakes on progressively older moraines provide baseline information for comparing successive levels of lake sediment cores from older moraines. Results indicate that the time of lake initiation seldom reflects moraine age. Even on the oldest moraine (Harris Creek), lake basins are presently forming. Their sediment character more closely relates to the rapidity of basin formation due to melting of buried ice than to age of the lake itself or of the moraine on which it is situated. Vegetation and soil development play an important but secondary role in determining the character of lake sediments; rapid subsidence can convert humic-water lakes surrounded by second-generation spruce forests into turbid-water lakes with unstable, slumping margins. A detailed paleolimnological study of two lakes, one on the unglaciated upland and another in an outwash channel penetrating the oldest moraine, revealed progressive limnologic changes through time, suggesting that their basins were stable for 1200 and 400 yr, respectively. The changes in diatom stratigraphy of these lakes appear to relate to natural limnological changes associated with lake maturation and accumulation of nutrients as well as to changes in the surrounding vegetation and soils.