Multichannel seismic reflection data are used, in conjunction with deep crustal seismic refraction data, to estimate the thickness of sediments in the southern Canada Basin of the Arctic Ocean north of Alaska. The sediments are interpreted to be of Hauterivian (mid-Early Cretaceous) to Holocene age. Comparison of the seismic reflection character of seismic reflections in the study area with that in other basins indicates that a base-of-sediment—top of oceanic layer 2 reflection is not present above the depth at which the water-bottom multiple obscures all deeper arrivals, which is in conflict with the conclusions drawn from aeromagnetic, refraction, and other reflection studies. Seismic velocity structure, determined from the reflection data, indicates that the reflections above the multiple are from sedimentary strata. In the absence of seismic reflection evidence for the top of layer 2 above the multiple, we estimate total sediment thickness by using the layer 3 refractions and subtracting an average assumed layer 2 thickness from the top of layer 3. Assuming that an average thickness of oceanic layer 2 (1.4 km) overlies layer 3 in the southern Canada Basin, sediment thickness in the study area is estimated to range between 6.5 km where water depth is 3.8 km to greater than 11 km where the water depth is 2 km. This is nearly double that of any previous estimates and should have a significant effect on calculations such as the age of Canada Basin, regional heat flow, and long-term sedimentation rates.