We have used anisotropy of magnetic susceptibility (AMS) to define the flow fabric and possible source area of the Peach Springs Tuff, a widespread rhyolitic ash flow tuff in the Mojave Desert and Great Basin of California, Arizona, and Nevada. The tuff is an important stratigraphic marker from the Colorado Plateau to Barstow, California, a distance of 350 km; however, the location of its source caldera is unknown. Dated at 18.5 Ma by ⁴⁰Ar/³⁹ Ar, the tuff erupted during the early stages of Miocene extension along the lower Colorado River. The thicker accumulations (>100 m) occur at Kingman, Arizona, and in the Piute Mountains, California, on opposite sides of the Colorado River extensional corridor. Our AMS studies produced well-defined magnetic lineations in 30 of 42 sites distributed throughout the tuff. Typical ratios of the principal AMS axes are 1.01 for the magnetic lineation (k_max/k_int) and 1.02 for the foliation (k_int/k_min); the bulk magnetic susceptibility of the Peach Springs Tuff averages 2.0×10⁻³ in the SI unit system. The subhorizontal lineations, which presumably parallel the flow directions, form a pattern radiating outward from the approximate center of the outcrop area. Magnetic foliations define an imbrication that generally dips away from the distal margins and toward the center of the outcrop of the tuff. The lineation and imbrication indicate a source region near the southern tip of Nevada. Defining the best intersection of the AMS lineations required restoration of major extension, strike-slip faulting, and associated tectonic rotation in the disrupted tuff. The optimum intersection of magnetic lineations lies in the southern Black Mountains of Arizona on the eastern side of the Colorado River extensional corridor. No caldera structures are known from that area, but the area contains thick sections of the Peach Springs Tuff above a silicic volcanic center. The caldera may be buried under younger deposits in the Mohave Valley of Arizona. Tertiary granite in the Newberry Mountains may represent a deeper level of the Peach Springs Tuff vent that has been exhumed by detachment faulting.