Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site
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Abstract
Pesticide concentrations in ground water at Rocky Mountain Arsenal (RMA) near Denver, Colorado, were determined using solid-phase extraction (SPE) gas chromatography/mass spectrometry (GC/MS) procedures and enzyme-linked immunosorbent assay (ELISA) for cyclodiene insecticides and triazine herbicides. Matrix interferences resulted in inconclusive results for some GC/MS analyses due to baseline disturbances and co-elution, but ELISA analyses consistently gave definitive results in a minimum amount of time. ELISA was used initially as a screening method, and pesticide concentrations and plume extents identified by ELISA were confirmed by SPE-GC/MS. A high degree of correlation was seen between results from GC/MS and ELISA methods for the triazine herbicides (correlation coefficient (R2) = 0.99). All areas with high pesticide concentrations were found to be within the boundaries of RMA.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site |
| Series title | ACS Symposium Series |
| DOI | 10.1021/bk-1997-0657.ch018 |
| Volume | 657 |
| Publication Date | July 23, 2009 |
| Year Published | 1996 |
| Language | English |
| Publisher | ACS Publications |
| Description | 13 p. |
| First page | 221 |
| Last page | 233 |
| Country | United States |
| State | Colorado |
| Other Geospatial | Rocky Mountain Arsenal |