Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site
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Abstract
Pesticide concentrations in ground water at Rocky Mountain Arsenal (RMA) near Denver, Colorado, were determined using solid-phase extraction (SPE) gas chromatography/mass spectrometry (GC/MS) procedures and enzyme-linked immunosorbent assay (ELISA) for cyclodiene insecticides and triazine herbicides. Matrix interferences resulted in inconclusive results for some GC/MS analyses due to baseline disturbances and co-elution, but ELISA analyses consistently gave definitive results in a minimum amount of time. ELISA was used initially as a screening method, and pesticide concentrations and plume extents identified by ELISA were confirmed by SPE-GC/MS. A high degree of correlation was seen between results from GC/MS and ELISA methods for the triazine herbicides (correlation coefficient (R2) = 0.99). All areas with high pesticide concentrations were found to be within the boundaries of RMA.
Suggested Citation
Dombrowski, T.R., Thurman, E., Mohrman, G.B., 1996, Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site: ACS Symposium Series, v. 657, p. 221-233, https://doi.org/10.1021/bk-1997-0657.ch018.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site |
| Series title | ACS Symposium Series |
| DOI | 10.1021/bk-1997-0657.ch018 |
| Volume | 657 |
| Publication Date | July 23, 2009 |
| Year Published | 1996 |
| Language | English |
| Publisher | ACS Publications |
| Description | 13 p. |
| First page | 221 |
| Last page | 233 |
| Country | United States |
| State | Colorado |
| Other Geospatial | Rocky Mountain Arsenal |