Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas)
Methods in Cell Science
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Abstract
Techniques are described for preparing, preserving, and growing cell cultures from 30 to 40-day old green turtle embryos (2.0-3.0 cm length) including cells derived from skeletal muscle, liver, heart, kidney, eye, lung, and brain. Acceptable growth of all cells occurred in all standard cell culture media tested, with optimum growth temperature near 30??C. These cell cultures will be used in the study of sea turtle viral diseases including fibropapillomatosis, which is currently epidemic in some green turtle populations.
Suggested Citation
Moore, M.K., Work, T.M., Balazs, G.H., Docherty, D., 1997, Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas): Methods in Cell Science, v. 19, no. 3, p. 161-168, https://doi.org/10.1023/A:1009716712646.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas) |
| Series title | Methods in Cell Science |
| DOI | 10.1023/A:1009716712646 |
| Volume | 19 |
| Issue | 3 |
| Year Published | 1997 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Contributing office(s) | National Wildlife Health Center |
| Description | 8 p. |
| First page | 161 |
| Last page | 168 |