Methods for assessing the impacts of ultraviolet-B radiation on aquatic invertebrates

ASTM Special Technical Publication STP1333
By: , and 
Edited by: Edward E. LittleBruce M. Greenberg, and Aaron J. Delonay



A standard methodology for assessing the impacts of simulated solar ultraviolet-B radiation (UV-B) on aquatic invertebrates was established. A solar simulator was used to expose a variety of aquatic invertebrates to different levels of UV-B. The simulator was calibrated as close as possible to match local ambient solar radiation measured in and out of water with a scanning spectroradiometer. A series of repeated exposures were conducted to determine the effects of UV-B on two species of Ceriodaphnia. Survivorship of C. reticulata declined with increasing UV-B with 100% mortality occurring after four daily 5 hr exposures to a UV-B irradiance that was 14% of ambient sunlight (40.8/μW/cm2) and 70% mortality for C. dubia after seven days of an exposure to 5% of ambient (14.5μW/cm2). Significant reductions in fertility (#young/adult) was observed in both low and high light adapted individuals with low light individuals appearing to be more sensitive. This methodology allowed us to make comparisons to natural conditions in aquatic habitats and to make risk assessments for individual species.

Publication type Report
Publication Subtype Organization Series
Title Methods for assessing the impacts of ultraviolet-B radiation on aquatic invertebrates
Series title ASTM Special Technical Publication
Series number STP1333
ISBN 978-0-8031-1485-2
DOI 10.1520/STP12153S
Volume 7
Year Published 1998
Language English
Publisher ASTM International
Contributing office(s) Columbia Environmental Research Center
Description 14 p.
Larger Work Type Report
Larger Work Subtype Organization Series
Larger Work Title Environmental toxicology and risk assessment: Seventh volume (STP1333)
First page 31
Last page 44
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