Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: Two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic
Archives of Microbiology
By: Blum J. Switzer, Bindi A. Burns, J. Buzzelli, J.F. Stolz, and R.S. Oremland
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Abstract
Two gram-positive anaerobic bacteria (strains E1H and MLS10) were isolated from the anoxic muds of Mono Lake, California, an alkaline, hypersaline, arsenic-rich water body. Both grew by dissimilatory reduction of As(V) to As(III) with the concomitant oxidation of lactate to acetate plus CO2. Bacillus arsenicoselenatis (strain E1H) is a spore-forming rod that also grew by dissimilatory reduction of Se(VI) to Se(IV). Bacillus selenitireducens (strain MLS 10) is a short, non-spore-forming rod that grew by dissimilatory reduction of Se(IV) to Se(0). When the two isolates were cocultured, a complete reduction of Se(VI) to Se(0) was achieved. Both isolates are alkaliphiles and had optimal specific growth rates in the pH range of 8.5-10. Strain E1H had a salinity optimum at 60 g 1-1 NaCl, while strain MLS10 had optimal growth at lower salinities (24-60 g 1-1 NaCl). Both strains have limited abilities to grow with electron donors and acceptors other than those given above. Strain MLS10 demonstrated weak growth as a microaerophile and was also capable of fermentative growth on glucose, while strain E1H is a strict anaerobe. Comparative 16S rRNA gene sequence analysis placed the two isolates with other Bacillus spp. in the low G+C gram-positive group of bacteria.
Suggested Citation
Switzer, B.J., Burns, B.A., Buzzelli, J., Stolz, J., Oremland, R., 1998, Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: Two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic: Archives of Microbiology, v. 171, no. 1, p. 19-30, https://doi.org/10.1007/s002030050673.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: Two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic |
| Series title | Archives of Microbiology |
| DOI | 10.1007/s002030050673 |
| Volume | 171 |
| Issue | 1 |
| Year Published | 1998 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Toxic Substances Hydrology Program |
| Description | 12 p. |
| First page | 19 |
| Last page | 30 |