Difluoromethane, a new and improved inhibitor of methanotrophy
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Abstract
Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation byMethylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and this inhibition was reversed by DFM removal. Although a small quantity of DFM was consumed during these incubations, its remaining concentration was sufficiently elevated to sustain inhibition. Methanogenesis in anaerobic soil slurries, including acetoclastic methanogenesis, was unaffected by levels of DFM which inhibit methanotrophy. Low levels of DFM (0.03 kPa) also inhibited nitrification and N2O production by soils. DFM is proposed as an improved inhibitor of CH4 oxidation over acetylene and/or methyl fluoride on the basis of its reversibility, its efficacy at low concentrations, its lack of inhibition of methanogenesis, and its low cost.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Difluoromethane, a new and improved inhibitor of methanotrophy |
| Series title | Applied and Environmental Microbiology |
| DOI | 10.1128/AEM.64.11.4357-4362.1998 |
| Volume | 64 |
| Issue | 11 |
| Year Published | 1998 |
| Language | English |
| Publisher | American Society for Microbiology |
| Contributing office(s) | Toxic Substances Hydrology Program |
| Description | 6 p. |
| First page | 4357 |
| Last page | 4362 |
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