Toxicity of o,p′-DDE to medaka d-rR strain after a one-time embryonic exposure by in ovo nanoinjection: An early through juvenile life cycle assessment
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Abstract
The toxicity of o,p′-DDE (1,1-dichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethylene) was evaluated in embryos of medaka (Oryzias latipes) following a one time exposure via nanoinjection. Medaka eggs (early gastrula) were injected with 0.5 nl of triolein (vehicle control) or 0.5 nl of 4 graded doses (0.0005-0.5 ng/egg) of o,p′-DDE in triolein. Embryos were allowed to develop, and fry were reared. Embryonic survival was monitored daily during the first 10 d until hatching and thereafter, on a weekly basis until day 59, at which time the fish were monitored for sexual maturity until day 107. In general, o,p′-DDE caused a dose- and time-dependent mortality. No changes in mortality were observed between the last two time points (day 38 and 59, respectively), and hence a 59 day-LD50 of 346 ng o,p′-DDE/egg was derived from the linear dose-response relationship. Prior to late stage death, only isolated cases of cardiovascular lesions and spinal deformities were observed, but were not dose-dependent. The lowest observable adverse effect level (LOAEL), based on upper 95% CI for regression line=0.0018 mg/kg, and the LOAEL based on exposure doses=0.5 mg/kg. Likewise, the no observable adverse effect level (NOAEL) based on linear extrapolation to 100% survival=0.0000388 mg/kg, while the NOAEL based on exposure doses=0.05 mg/kg. The nanoinjection medaka model has potential in the study of hormonally active compounds in the environment.
Suggested Citation
Villalobos, S.A., Papoulias, D.M., Pastva, S.D., Blankenship, A.L., Meadows, J.C., Tillitt, D.E., Giesy, J.P., 2003, Toxicity of o,p′-DDE to medaka d-rR strain after a one-time embryonic exposure by in ovo nanoinjection: An early through juvenile life cycle assessment: Chemosphere, v. 53, no. 8, p. 819-826, https://doi.org/10.1016/S0045-6535(03)00583-6.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Toxicity of o,p′-DDE to medaka d-rR strain after a one-time embryonic exposure by in ovo nanoinjection: An early through juvenile life cycle assessment |
| Series title | Chemosphere |
| DOI | 10.1016/S0045-6535(03)00583-6 |
| Volume | 53 |
| Issue | 8 |
| Year Published | 2003 |
| Language | English |
| Publisher | Elsevier |
| Description | 8 p. |
| First page | 819 |
| Last page | 826 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |