Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction
Journal of Wildlife Diseases
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Abstract
We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C. botulinum type C cells in tilapia gastrointestinal contents at a level of 7.5×104 cells per 0.25 g material or 1.9×103 cells. This technique is useful for determining prevalence of the potentially active organisms within a given population of fish and may be adapted to other types of C. botulinum and vertebrate populations as well.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/0090-3558-40.4.749 |
| Volume | 40 |
| Issue | 4 |
| Year Published | 2004 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | National Wildlife Health Center |
| Description | 5 p. |
| First page | 749 |
| Last page | 753 |
| Country | United States |
| State | California |
| Other Geospatial | Salton Sea |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |