In 2002 and 2003 we released a total of 66 hatchery-reared, juvenile paddlefish Polyodon spathula (249-318 mm eye-to-fork length) in Pennsylvania's upper Ohio River system and tracked them with radiotelemetry in two different pools of the Ohio and Allegheny rivers to determine (1) poststocking survival, (2) whether release site influences survival, (3) dispersal distance and direction of movement, and (4) habitat selection. Survival was fair (mean = 78% in 2002 and 67% in 2003) for 0.23-0.43-kg paddlefish after 9 weeks. In 2003, fish stocked in the upstream half of the pool had a greater survival (100%) after 63 d than those stocked in the downstream half (44%). Within 4 d of stocking, 77% of juvenile paddlefish were located in tailwaters, and fish found these habitats regardless of stocking location. Habitat measurements at all postdispersal locations had median depths of 5.2 and 6.1 m in 2002 and 2003, respectively, and median near-surface velocities of 0.17 and 0.12 m/s. Fish selected tailwater habitats and avoided habitats with disturbance from commercial barge traffic in both years. ?? Copyright by the American Fisheries Society 2007.