Molecular and biochemical analysis of rainbow trout LCK suggests a conserved mechanism for T-cell signaling in gnathostomes
Molecular Immunology
By: K.J. Laing, S. Dutton, and J.D. Hansen
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Abstract
Two genes were identified in rainbow trout that display high sequence identity to vertebrate Lck. Both of the trout Lck transcripts are associated with lymphoid tissues and were found to be highly expressed in IgM-negative lymphocytes. In vitro analysis of trout lymphocytes indicates that trout Lck mRNA is up-regulated by T-cell mitogens, supporting an evolutionarily conserved function for Lck in the signaling pathways of T-lymphocytes. Here, we describe the generation and characterization of a specific monoclonal antibody raised against the N-terminal domains of recombinant trout Lck that can recognize Lck protein(s) from trout thymocyte lysates that are similar in size (???57 kDa) to mammalian Lck. This antibody also reacted with permeabilized lymphocytes during FACS analysis, indicating its potential usage for cellular analyses of trout lymphocytes, thus representing an important tool for investigations of salmonid T-cell function.
Suggested Citation
Laing, K., Dutton, S., and Hansen, J., 2007, Molecular and biochemical analysis of rainbow trout LCK suggests a conserved mechanism for T-cell signaling in gnathostomes: Molecular Immunology, v. 44, no. 10, p. 2737-2748, https://doi.org/10.1016/j.molimm.2006.11.021.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Molecular and biochemical analysis of rainbow trout LCK suggests a conserved mechanism for T-cell signaling in gnathostomes |
| Series title | Molecular Immunology |
| DOI | 10.1016/j.molimm.2006.11.021 |
| Volume | 44 |
| Issue | 10 |
| Year Published | 2007 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Molecular Immunology |
| First page | 2737 |
| Last page | 2748 |