Natural calcium isotonic composition of urine as a marker of bone mineral balance
Clinical Chemistry
By: J. Skulan, T. Bullen, A.D. Anbar, J.E. Puzas, L. Shackelford, A. LeBlanc, and S. M. Smith
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Abstract
Background: We investigated whether changes in the natural isotopic composition of calcium in human urine track changes in net bone mineral balance, as predicted by a model of calcium isotopic behavior in vertebrates. If so, isotopic analysis of natural urine or blood calcium could be used to monitor short-term changes in bone mineral balance that cannot be detected with other techniques. Methods: Calcium isotopic compositions are expressed as ??44Ca, or the difference in parts per thousand between the 44Ca/40Ca of a sample and the 44Ca/ 40Ca of a standard reference material. ??44Ca was measured in urine samples from 10 persons who participated in a study of the effectiveness of countermeasures to bone loss in spaceflight, in which 17 weeks of bed rest was used to induce bone loss. Study participants were assigned to 1 of 3 treatment groups: controls received no treatment, one treatment group received alendronate, and another group performed resistive exercise. Measurements were made on urine samples collected before, at 2 or 3 points during, and after bed rest. Results: Urine ??44Ca values during bed rest were lower in controls than in individuals treated with alendronate (P <0.05, ANOVA) or exercise (P <0.05), and lower than the control group baseline (P <0.05, Mest). Results were consistent with the model and with biochemical and bone mineral density data. Conclusion: Results confirm the predicted relationship between bone mineral balance and calcium isotopes, suggesting that calcium isotopic analysis of urine might be refined into a clinical and research tool. ?? 2007 American Association for Clinical Chemistry.
Suggested Citation
Skulan, J., Bullen, T., Anbar, A., Puzas, J., Shackelford, L., LeBlanc, A., Smith, S.M., 2007, Natural calcium isotonic composition of urine as a marker of bone mineral balance: Clinical Chemistry, v. 53, no. 6, p. 1155-1158, https://doi.org/10.1373/clinchem.2006.080143.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Natural calcium isotonic composition of urine as a marker of bone mineral balance |
| Series title | Clinical Chemistry |
| DOI | 10.1373/clinchem.2006.080143 |
| Volume | 53 |
| Issue | 6 |
| Publication Date | June 01, 2007 |
| Year Published | 2007 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Clinical Chemistry |
| First page | 1155 |
| Last page | 1158 |