An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress
Insect Molecular Biology
By: N. K. Karouna-Renier and K.R. Rao
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Abstract
In the present study, we identified and characterized an inducible heat shock protein 70 (HSP70) from the midge Chironomus dilutus and investigated the transcriptional profile of the gene under baseline and environmentally stressful conditions. Using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), we observed increased expression of CD-HSP70-1 in response to both heat shock and copper stress. We also investigated the expression of this gene during midge development. All C. dilutus developmental stages expressed CD-HSP70-1 under normal conditions, although at extremely low levels. Phylogenetic analysis of the amino acid sequence demonstrated distinct clustering of this gene with inducible HSP70s from other insect species. ?? 2008 The Authors.
Suggested Citation
Karouna-Renier, N.K., Rao, K., 2009, An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress: Insect Molecular Biology, v. 18, no. 1, p. 87-96, https://doi.org/10.1111/j.1365-2583.2008.00853.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress |
| Series title | Insect Molecular Biology |
| DOI | 10.1111/j.1365-2583.2008.00853.x |
| Volume | 18 |
| Issue | 1 |
| Publication Date | January 19, 2009 |
| Year Published | 2009 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Insect Molecular Biology |
| First page | 87 |
| Last page | 96 |