Tetranucleotide microsatellite loci from the black bear (Ursus americanus)
Molecular Ecology Resources
By: J.S. Sanderlin, B.C. Faircloth, B. Shamblin, and M.J. Conroy
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Abstract
We describe primers and polymerase chain reaction conditions to amplify 21 tetranucleotide microsatellite DNA loci in black bears (Ursus americanus). We tested primers using individuals from two populations, one each in Georgia and Florida. Among individuals from Georgia (n = 29), primer pairs yielded an average of 2.9 alleles (range, one to four) and an average observed heterozygosity (HO) of 0.50 (range, 0.00 to 0.79). Among individuals from Florida (n = 19), primer pairs yielded an average of 5.7 alleles (range, one to 14) and an HO of 0.55 (range, 0.00 to 1.00). A comparison of previously developed markers with individuals from Georgia suggests that bear populations in Georgia and Florida have reduced allelic diversity relative to other populations. ?? 2008 The Authors.
Suggested Citation
Sanderlin, J., Faircloth, B., Shamblin, B., Conroy, M., 2009, Tetranucleotide microsatellite loci from the black bear (Ursus americanus): Molecular Ecology Resources, v. 9, no. 1, p. 288-291, https://doi.org/10.1111/j.1755-0998.2008.02234.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Tetranucleotide microsatellite loci from the black bear (Ursus americanus) |
| Series title | Molecular Ecology Resources |
| DOI | 10.1111/j.1755-0998.2008.02234.x |
| Volume | 9 |
| Issue | 1 |
| Publication Date | January 22, 2009 |
| Year Published | 2009 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Molecular Ecology Resources |
| First page | 288 |
| Last page | 291 |