Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
Molecular Ecology Resources
By: M.S. Hoy, K. Kelly, and R. J. Rodriguez
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Abstract
A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the binding sites for the universal primers resulting in the amplification of two products from the target species and one product from the nontarget species. Therefore, nontarget products are positive amplification controls. The 3-primer system accurately discriminated zebra and quagga mussels from seven geographically distinct populations.
Suggested Citation
Hoy, M., Kelly, K., Rodriguez, R.J., 2010, Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel): Molecular Ecology Resources, v. 10, no. 1, p. 190-192, https://doi.org/10.1111/j.1755-0998.2009.02727.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel) |
| Series title | Molecular Ecology Resources |
| DOI | 10.1111/j.1755-0998.2009.02727.x |
| Volume | 10 |
| Issue | 1 |
| Publication Date | December 16, 2009 |
| Year Published | 2010 |
| Language | English |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Molecular Ecology Resources |
| First page | 190 |
| Last page | 192 |