Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)
Molecular Ecology Resources
By: D. Ramirez-Macias, K. Shaw, R. Ward, F. Galvan-Magana, and R. Vazquez-Juarez
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Abstract
In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy–Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications.
Suggested Citation
Ramirez-Macias, D., Shaw, K., Ward, R., Galvan-Magana, F., Vazquez-Juarez, R., 2009, Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus): Molecular Ecology Resources, v. 9, no. 3, p. 798-800, https://doi.org/10.1111/j.1755-0998.2008.02197.x.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus) |
| Series title | Molecular Ecology Resources |
| DOI | 10.1111/j.1755-0998.2008.02197.x |
| Volume | 9 |
| Issue | 3 |
| Publication Date | April 20, 2009 |
| Year Published | 2009 |
| Language | English |
| Publisher | Wiley |
| Contributing office(s) | Leetown Science Center |
| Description | 3 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Molecular Ecology Resources |
| First page | 798 |
| Last page | 800 |