A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth

M.K. Choi; C. H. Moon; M.S. Ko; U.-H. Lee; W. Cho; S.J. Cha; J.W. Do; G.J. Heo; S.G. Jeong; Y.S. Hahm; A. Harmache; M. Bremont; Gael Kurath; J. W. Park

doi:10.1371/journal.pone.0022362

A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth

PLoS ONE

By: M.K. Choi, C. H. Moon, M.S. Ko, U.-H. Lee, W. Cho, S.J. Cha, J.W. Do, G.J. Heo, S.G. Jeong, Y.S. Hahm, A. Harmache, M. Bremont, Gael Kurath, and J. W. Park

https://doi.org/10.1371/journal.pone.0022362

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Abstract

The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity of IHNV. However, little is known about the mechanism by which the NV supports the viral growth. In this study, cellular localization of NV and its role in IHNV growth in host cells was investigated. Through transient transfection in RTG-2 cells of NV fused to green fluorescent protein (GFP), a nuclear localization of NV was demonstrated. Deletion analyses showed that the ³²EGDL³⁵ residues were essential for nuclear localization of NV protein, and fusion of these 4 amino acids to GFP directed its transport to the nucleus. We generated a recombinant IHNV, rIHNV-NV-ΔEGDL in which the ³²EGDL³⁵ was deleted from the NV. rIHNVs with wild-type NV (rIHNV-NV) or with the NV gene replaced with GFP (rIHNV-ΔNV-GFP) were used as controls. RTG-2 cells infected with rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL yielded 12- and 5-fold less infectious virion, respectively, than wild type rIHNV-infected cells at 48 h post-infection (p.i.). While treatment with poly I:C at 24 h p.i. did not inhibit replication of wild-type rIHNVs, replication rates of rIHNV-ΔNV-GFP and rIHNV-NV-ΔEGDL were inhibited by poly I:C. In addition, both rIHNV-ΔNV and rIHNV-NV-ΔEGDL induced higher levels of expressions of both IFN1 and Mx1 than wild-type rIHNV. These data suggest that the IHNV NV may support the growth of IHNV through inhibition of the INF system and the amino acid residues of ³²EGDL³⁵ responsible for nuclear localization are important for the inhibitory activity of NV.

Suggested Citation

Choi, M., Moon, C.H., Ko, M., Lee, U., Cho, W., Cha, S., Do, J., Heo, G., Jeong, S., Hahm, Y., Harmache, A., Bremont, M., Kurath, G., and Park, J.W., 2011, A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth: PLoS ONE, v. 6, no. 7, Article ID: e22362, https://doi.org/10.1371/journal.pone.0022362.

Additional publication details
Publication type	Article
Publication Subtype	Journal Article
Title	A nuclear localization of the infectious haematopoietic necrosis virus NV protein is necessary for optimal viral growth
Series title	PLoS ONE
DOI	10.1371/journal.pone.0022362
Volume	6
Issue	7
Publication Date	July 21, 2011
Year Published	2011
Language	English
Publisher	PLOS
Publisher location	San Francisco, CA
Contributing office(s)	Western Fisheries Research Center
Description	Article ID: e22362
Larger Work Type	Article
Larger Work Subtype	Journal Article
Larger Work Title	PLoS ONE