Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting
Journal of Aquatic Animal Health
By: Maureen K. Purcell, Rodman G. Getchell, Carol A. McClure, S.E. Weber, and Kyle A. Garver
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Abstract
Real-time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other molecular methods for detecting the nucleic acids of human and other animal pathogens owing to the speed and robustness of the technology. As the aquatic animal health community moves toward implementing national diagnostic testing schemes, it will need to evaluate how qPCR technology should be employed. This review outlines the basic principles of qPCR technology, considerations for assay development, standards and controls, assay performance, diagnostic validation, implementation in the diagnostic laboratory, and quality assurance and control measures. These factors are fundamental for ensuring the validity of qPCR assay results obtained in the diagnostic laboratory setting.
Suggested Citation
Purcell, M., Getchell, R.G., McClure, C.A., Weber, S., and Garver, K.A., 2011, Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting: Journal of Aquatic Animal Health, v. 23, no. 3, p. 148-161, https://doi.org/10.1080/08997659.2011.620217.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting |
| Series title | Journal of Aquatic Animal Health |
| DOI | 10.1080/08997659.2011.620217 |
| Volume | 23 |
| Issue | 3 |
| Publication Date | October 10, 2011 |
| Year Published | 2011 |
| Language | English |
| Publisher | Taylor & Francis |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 14 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Journal of Aquatic Animal Health |
| First page | 148 |
| Last page | 161 |