TanA: a fluorogenic probe for thiaminase activity
Analytical Methods
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Abstract
A fluorogenic thiamine analogue is presented as a fluorescent probe for thiaminase activity. The emission of the fluorophore is quenched by photoinduced electron transfer (PET) to the N-substituted pyridinium portion of the probe. Action of the enzyme releases the free pyridine group causing a substantial increase in fluorescence.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | TanA: a fluorogenic probe for thiaminase activity |
| Series title | Analytical Methods |
| DOI | 10.1039/C2AY26047F |
| Volume | 5 |
| Issue | 2 |
| Year Published | 2013 |
| Language | English |
| Publisher | RSC Publishing |
| Contributing office(s) | Columbia Environmental Research Center |
| Description | 3 p. |
| First page | 446 |
| Last page | 448 |