Correlation of gene expression and contaminat concentrations in wild largescale suckers: a field-based study
Science of the Total Environment
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Abstract
Toxic compounds such as organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ether flame retardants (PBDEs) have been detected in fish, birds, and aquatic mammals that live in the Columbia River or use food resources from within the river. We developed a custom microarray for largescale suckers (Catostomus macrocheilus) and used it to investigate the molecular effects of contaminant exposure on wild fish in the Columbia River. Using Significance Analysis of Microarrays (SAM) we identified 72 probes representing 69 unique genes with expression patterns that correlated with hepatic tissue levels of OCs, PCBs, or PBDEs. These genes were involved in many biological processes previously shown to respond to contaminant exposure, including drug and lipid metabolism, apoptosis, cellular transport, oxidative stress, and cellular chaperone function. The relation between gene expression and contaminant concentration suggests that these genes may respond to environmental contaminant exposure and are promising candidates for further field and laboratory studies to develop biomarkers for monitoring exposure of wild fish to contaminant mixtures found in the Columbia River Basin. The array developed in this study could also be a useful tool for studies involving endangered sucker species and other sucker species used in contaminant research.
Suggested Citation
Christiansen, H.E., Mehinto, A.C., Yu, F., Perry, R.W., Denslow, N., Maule, A.G., Mesa, M.G., 2014, Correlation of gene expression and contaminat concentrations in wild largescale suckers: a field-based study: Science of the Total Environment, v. 484, p. 379-389, https://doi.org/10.1016/j.scitotenv.2013.08.034.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Correlation of gene expression and contaminat concentrations in wild largescale suckers: a field-based study |
| Series title | Science of the Total Environment |
| DOI | 10.1016/j.scitotenv.2013.08.034 |
| Volume | 484 |
| Year Published | 2014 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 11 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Science of the Total Environment |
| First page | 379 |
| Last page | 389 |
| Country | United States |
| State | Oregon;Washington |
| Other Geospatial | Columbia River |