A rapid field test for sylvatic plague exposure in wild animals
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Abstract
Plague surveillance is routinely conducted to predict future epizootics in wildlife and exposure risk for humans. The most common surveillance method for sylvatic plague is detection of antibodies to Yersinia pestis F1 capsular antigen in sentinel animals, such as coyotes (Canis latrans). Current serologic tests for Y. pestis, hemagglutination (HA) test and enzyme-linked immunosorbent assay (ELISA), are expensive and labor intensive. To address this need, we developed a complete lateral flow device for the detection of specific antibodies to Y. pestis F1 and V antigens. Our test detected anti-F1 and anti-V antibodies in serum and Nobuto filter paper samples from coyotes, and in serum samples from prairie dogs (Cynomys ludovicianus), lynx (Lynx canadensis), and black-footed ferrets (Mustela nigripes). Comparison of cassette results for anti-F1 and anti-V antibodies with results of ELISA or HA tests showed correlations ranging from 0.68 to 0.98. This device provides an affordable, user-friendly tool that may be useful in plague surveillance programs and as a research tool.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A rapid field test for sylvatic plague exposure in wild animals |
| Series title | Journal of Wildlife Diseases |
| DOI | 10.7589/2013-07-174 |
| Volume | 50 |
| Issue | 2 |
| Year Published | 2014 |
| Language | English |
| Publisher | Wildlife Disease Association |
| Contributing office(s) | National Wildlife Health Center |
| Description | 5 p. |
| First page | 384 |
| Last page | 388 |
| Country | United States |
| State | Colorado, Montana, New Mexico |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |