Development and evaluation of a blocking enzyme-linked immunosorbent assay and virus neutralization assay to detect antibodies to viral hemorrhagic septicemia virus
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Abstract
Viral hemorrhagic septicemia virus (VHSV) is a target of surveillance by many state and federal agencies in the United States. Currently, the detection of VHSV relies on virus isolation, which is lethal to fish and indicates only the current infection status. A serological method is required to ascertain prior exposure. Here, we report two serologic tests for VHSV that are nonlethal, rapid, and species independent, a virus neutralization (VN) assay and a blocking enzyme-linked immunosorbent assay (ELISA). The results show that the VN assay had a specificity of 100% and sensitivity of 42.9%; the anti-nucleocapsid-blocking ELISA detected nonneutralizing VHSV antibodies at a specificity of 88.2% and a sensitivity of 96.4%. The VN assay and ELISA are valuable tools for assessing exposure to VHSV.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development and evaluation of a blocking enzyme-linked immunosorbent assay and virus neutralization assay to detect antibodies to viral hemorrhagic septicemia virus |
| Series title | Clinical and Vaccine Immunology |
| DOI | 10.1128/CVI.00675-13 |
| Volume | 21 |
| Issue | 3 |
| Publication Date | January 15, 2014 |
| Year Published | 2014 |
| Language | English |
| Publisher | American Society for Microbiology |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 8 p. |
| First page | 435 |
| Last page | 442 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |