Identification of Mn(II)-oxidizing bacteria from a low-pH contaminated former uranium mine
Applied and Environmental Microbiology
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Abstract
Biological Mn oxidation is responsible for producing highly reactive and abundant Mn oxide phases in the environment that can mitigate metal contamination. However, little is known about Mn oxidation in low-pH environments, where metal contamination often is a problem as the result of mining activities. We isolated two Mn(II)-oxidizing bacteria (MOB) at pH 5.5 (Duganella isolate AB_14 and Albidiferax isolate TB-2) and nine strains at pH 7 from a former uranium mining site. Isolate TB-2 may contribute to Mn oxidation in the acidic Mn-rich subsoil, as a closely related clone represented 16% of the total community. All isolates oxidized Mn over a small pH range, and isolates from low-pH samples only oxidized Mn below pH 6. Two strains with different pH optima differed in their Fe requirements for Mn oxidation, suggesting that Mn oxidation by the strain found at neutral pH was linked to Fe oxidation. Isolates tolerated Ni, Cu, and Cd and produced Mn oxides with similarities to todorokite and birnessite, with the latter being present in subsurface layers where metal enrichment was associated with Mn oxides. This demonstrates that MOB can be involved in the formation of biogenic Mn oxides in both moderately acidic and neutral pH environments.
Suggested Citation
Akob, D.M., Bohu, T., Beyer, A., Schaffner, F., Handel, M., Johnson, C., Merten, D., Büchel, G., Totsche, K., and Küsel, K., 2014, Identification of Mn(II)-oxidizing bacteria from a low-pH contaminated former uranium mine: Applied and Environmental Microbiology, v. 80, no. 16, p. 5086-5097, https://doi.org/10.1128/AEM.01296-14.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Identification of Mn(II)-oxidizing bacteria from a low-pH contaminated former uranium mine |
| Series title | Applied and Environmental Microbiology |
| DOI | 10.1128/AEM.01296-14 |
| Volume | 80 |
| Issue | 16 |
| Year Published | 2014 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher location | Washington, D.C. |
| Contributing office(s) | National Research Program - Eastern Branch |
| Description | 12 p. |
| Larger Work Type | Article |
| Larger Work Subtype | Journal Article |
| Larger Work Title | Applied and Environmental Microbiology |
| First page | 5086 |
| Last page | 5097 |
| Country | Germany |