Physical characteristics and persistence of macrohabitat used by different life stages of Sonora chub (Gila ditaenia) were determined by repeatedly measuring distinct reaches in Sycamore Creek, Santa Cruz County, Arizona, in 1990 and 1991. At the beginning of summer drought, habitats occupied by adult Sonora chub were deeper and larger than areas with only immature fish and unoccupied areas. The medians of maximum depth were 47.0 cm (1990) and 39.7 cm (1991) for habitats with adults, 21.3 cm (1990) and 22.9 cm (1991) for habitats with only immature fish, and 14.6 cm (1990) and 19.7 cm (1991) for unoccupied areas. At the end of summer drought, adults occupied habitats that were deeper and larger, and the percent decrease in area and depth was less than areas containing only immature fish or no fish. The medians of percent decrease in maximum depth were 13% (1990) and 21% (1991) for habitats with adults, 48% (1990) and 41% (1991) for habitats with only immature fish, and 42% (1990) and 33% (1991) for unoccupied areas. By the end of summer drought, habitats with only immature fish were not physically different from unoccupied areas. Loss of total surface area was highest in reaches that contained only immature fish or no fish (range = 36% to 94%). Most Sonora chub lost from evaporating surface waters were immature fish. Ephemeral and unoccupied areas had higher percentages of floating cover and coarser substrates than persistent, occupied areas.