A ribonuclease protection assay can distinguish spring viremia of carp virus from pike fry rhabdovirus

Bulletin of the European Association of Fish Pathologists
By: , and 

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Abstract

Thirteen rhabdovirus isolates from 10 teleost fish species as well as reference strains of spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV) cross-reacted in an indirect immunofluorescence assay and were thus indistinguishable by this method. A ribonuclease protection assay (RPA) using a super(32)P-labeled RNA probe made from a cloned copy of the full length SVCV glycoprotein (G) gene was able to discriminate clearly between the type strains of SVCV and PFRV and among the 13 rhabdovirus isolates. Results for the RPA were generally in agreement with standard serum neutralisation assays; however, the RPA was also able to detect genomic differences between isolates of SVCV. These results have implications for fish disease control programs for SVCV.

Publication type Article
Publication Subtype Journal Article
Title A ribonuclease protection assay can distinguish spring viremia of carp virus from pike fry rhabdovirus
Series title Bulletin of the European Association of Fish Pathologists
Volume 18
Issue 6
Year Published 1998
Language English
Publisher European Association of Fish Pathologists;
Contributing office(s) Western Fisheries Research Center
Description 5 p.
First page 220
Last page 224
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