The dose-dependent effect on protection and humoral response to a DNA vaccine against Infectious Hematopoietic Necrosis (IHN) virus in subyearling rainbow trout
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
A dose–response study that used the DNA vaccine pIHNw-G against infectious hematopoietic necrosis virus (IHNV) showed that complete and highly significant (P < 0.001) protection against a virus injection challenge can be attained in subyearling rainbow trout Oncorhynchus mykiss (145–160 g, 8- to 10-months-old) 6 weeks after a single intramuscular injection with doses as low as 1 μg. Complete protection was also reproducibly demonstrated at higher vaccine doses; however, no protection was observed with a 0.1-μg dose. Virus-neutralizing antibody titers were detected in fish that had been vaccinated with different doses of the DNA vaccine and then sham-infected; there appeared to be a dose-dependent effect, with higher titers obtained with higher doses of vaccine. The DNA-vaccinated animals that survived virus challenge had significantly (P < 0.05) higher neutralizing antibody titers than sham-infected, DNA-vaccinated control fish. Additionally, the titers detected in the IHN survivors exhibited a significant (P < 0.05) dose-dependent effect, with the highest titers being present in fish that received the highest vaccine doses.
Suggested Citation
LaPatra, S.E., Corbeil, S., Jones, G.R., Shewmaker, W.D., and Kurath, G., 2000, The dose-dependent effect on protection and humoral response to a DNA vaccine against Infectious Hematopoietic Necrosis (IHN) virus in subyearling rainbow trout: Journal of Fish Diseases, v. 12, no. 3, p. 181-188, https://doi.org/10.1577/1548-8667(2000)012<0181:FDDEOP>2.0.CO;2.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | The dose-dependent effect on protection and humoral response to a DNA vaccine against Infectious Hematopoietic Necrosis (IHN) virus in subyearling rainbow trout |
| Series title | Journal of Fish Diseases |
| DOI | 10.1577/1548-8667(2000)012<0181:FDDEOP>2.0.CO;2 |
| Volume | 12 |
| Issue | 3 |
| Year Published | 2000 |
| Language | English |
| Publisher | Taylor & Francis |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 8 p. |
| First page | 181 |
| Last page | 188 |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |