An improved method for isolating viruses from asymptomatic carrier fish
Transactions of the American Fisheries Society
By: Donald F. Amend and John P. Pietsch
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Abstract
This paper describes a method using elevated levels of penicillin, streptomycin, and nystatin instead of filters to control bacteria and mold contaminants in specimens processed for virus isolation. Filters were shown to significantly reduce the virus concentration. Virus and tissue cultures were not affected by this procedure. In field tests nearly three times more specimens were positive for virus with this method than with the widely used filter technique. Moreover, the cost of materials was less. This method is recommended for inspection and certification purposes.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | An improved method for isolating viruses from asymptomatic carrier fish |
| Series title | Transactions of the American Fisheries Society |
| DOI | 10.1577/1548-8659(1972)101<267:AIMFIV>2.0.CO;2 |
| Volume | 101 |
| Issue | 2 |
| Year Published | 1972 |
| Language | English |
| Publisher | Taypr & Francis |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 3 p. |
| First page | 267 |
| Last page | 269 |
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