The nature of carbon (C) cycling in the unsaturated zone where groundwater is in contact with abundant gas-filled voids is poorly understood. The objective of this study was to trace inorganic-C cycling in a karst landscape using stable-C isotopes, with emphasis on a shallow groundwater flow path through the soil, to an underlying cave, and to the spring outlet of a cave stream in the Ozark Plateaus of northwestern Arkansas. Carbon dioxide (CO₂) concentration and isotopic composition (δ¹³C-CO₂) in gas and dissolved inorganic carbon (DIC) concentration and isotopic composition (δ¹³C-DIC) in water were measured in samples collected from two suction-cup soil samplers above the cave, three sites in the cave, and at the spring outlet of the cave stream. Soil-gas CO₂ concentration (median 2,578 ppm) and δ¹³C-CO₂ (median − 21.5‰) were seasonally variable, reflecting the effects of surface temperature changes on soil-CO₂ production via respiration and organic-matter decomposition. Cave-air CO₂ (median 1,026 ppm) was sourced from the soil zone and the surface atmosphere, with seasonally changing proportions of each source controlled by surface temperature-driven air density gradients. Soil-DIC concentration (median 1.7 mg L^− 1) was lower and soil-δ¹³C-DIC (median − 19.5‰) was lighter compared to the cave (median 23.3 mg L^− 1 and − 14.3‰, respectively) because carbonate-bedrock dissolution provided an inorganic source of C to the cave. Carbon species in the soil had a unique, light stable-C isotopic signature compared to the cave. Discrimination of soil-C sources to karst groundwater was achieved, which is critical for developing hydrologic budgets using environmental tracers such as C.