Large-scale dialysis of sample lipids
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
The use of a semipermeable membrane device (SPMD) for dialysis in an organic solvent phase is an efficient alternative approach to separation of contaminants from large amounts of lipid (up to 50 grams or more) prior to organic chemical analysis. Passive separation of contaminants can be accomplished with a minimum of equipment and a comparatively small volume of solvent. This study examines the effects of factors such as dialytic solvent, lipid type, dialytic solvent:lipid volume ratio, dialysis time, and temperature on the performance of polyethylene SPMDs during lipid-contaminant separations. The experimental conditions for maximal recoveries of organochlorine pesticides and polychlorinated biphenyls with minimal lipid carryover are determined for the examined variables. When the dialytic procedure is optimized, very satisfactory and highly reproducible analyte recoveries can be obtained in a few days while separating > 90% of the lipid material in a single operation.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Large-scale dialysis of sample lipids |
| Series title | Chemosphere |
| DOI | 10.1016/0045-6535(93)90026-2 |
| Volume | 26 |
| Issue | 11 |
| Year Published | 1993 |
| Language | English |
| Publisher | Elsevier |
| Contributing office(s) | Columbia Environmental Research Center |
| Description | 14 p. |
| First page | 1993 |
| Last page | 2006 |
| Country | United States |
| Google Analytic Metrics | Metrics page |