A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
Links
- More information: Publisher Index Page (via DOI)
- Data Release: USGS data release - Novel protocol for moose DNA extraction from Tempus blood tube supernatant following RNA extraction
- Open Access Version: Publisher Index Page
- Download citation as: RIS | Dublin Core
Abstract
Objective
We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.
Results
Of the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4–337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1–9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.
Suggested Citation
Ferrante, J.A., Giles, M.R., Benzie, E., Hunter, M., 2018, A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation: BMC Research Notes, v. 11, p. 1-5, https://doi.org/10.1186/s13104-018-3671-4.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation |
| Series title | BMC Research Notes |
| DOI | 10.1186/s13104-018-3671-4 |
| Volume | 11 |
| Publication Date | August 06, 2018 |
| Year Published | 2018 |
| Language | English |
| Publisher | BMC |
| Contributing office(s) | Wetland and Aquatic Research Center |
| Description | Article 563; 5 p. |
| First page | 1 |
| Last page | 5 |