A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
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Abstract
Objective
We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel.
Results
Of the 23 samples analyzed, the average DNA concentration was 121 ng/µl (range 4–337 ng/µl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1–9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation |
| Series title | BMC Research Notes |
| DOI | 10.1186/s13104-018-3671-4 |
| Volume | 11 |
| Year Published | 2018 |
| Language | English |
| Publisher | BMC |
| Contributing office(s) | Wetland and Aquatic Research Center |
| Description | Article 563; 5 p. |
| First page | 1 |
| Last page | 5 |
| Google Analytic Metrics | Metrics page |