Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America
Conservation Genetics Resources
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Abstract
We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America |
| Series title | Conservation Genetics Resources |
| DOI | 10.1007/s12686-019-01118-7 |
| Volume | 12 |
| Year Published | 2020 |
| Language | English |
| Publisher | Springer |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 3 p. |
| First page | 361 |
| Last page | 363 |
| Country | United States |
| Other Geospatial | Pacific Northwest |
| Google Analytic Metrics | Metrics page |