Functional characterization and osmoregulatory role of the Na+/K+/2Cl--cotransporter (NKCC1) in the gill of sea lamprey (Petromyzon marinus), a basal vertebrate
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
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Abstract
The present study provides molecular and functional characterization of Na+/K+/2Cl- cotransporter (nkcc1/NKCC1) in the gills of sea lamprey, the most basal extant vertebrate with an osmoregulatory strategy. We report the full-length peptide sequence for the lamprey NKCC1, which we show to group strongly with and occupy a basal position among other vertebrate NKCC1 sequences. Lamprey nkcc1 mRNA were present in many tissues but was 5-fold higher in the gill than any other tissue. NKCC1 protein was only detected in the gill. Gill mRNA and protein abundances of NKCC1 and Na+/K+-ATPase (NKA) were significantly upregulated (20- to 200-fold) in late metamorphosis in freshwater, coinciding with the development of salinity tolerance, and were upregulated an additional 2-fold after acclimation to seawater. Immunohistochemistry revealed that NKCC1 in the gill is found in filamental ionocytes that develop during metamorphosis. Lamprey treated with bumetanide, a widely used pharmacological inhibitor of NKCC1, exhibited higher plasma Cl- and osmolality and reduced muscle water content after 24 h in seawater, but had no effect in FW. This work provides the first functional characterization of NKCC1 as having a functional role mechanism for branchial salt secretion in lampreys, providing evidence that this mode of Cl- secretion has been present among vertebrates for ~550 million years.
Suggested Citation
Shaughnessy, C.A., McCormick, S.D., 2019, Functional characterization and osmoregulatory role of the Na+/K+/2Cl--cotransporter (NKCC1) in the gill of sea lamprey (Petromyzon marinus), a basal vertebrate: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, v. 318, no. 1, p. R17-R29, https://doi.org/10.1152/ajpregu.00125.2019.
ISSN: 1522-1490 (online)
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Functional characterization and osmoregulatory role of the Na+/K+/2Cl--cotransporter (NKCC1) in the gill of sea lamprey (Petromyzon marinus), a basal vertebrate |
| Series title | American Journal of Physiology - Regulatory, Integrative and Comparative Physiology |
| DOI | 10.1152/ajpregu.00125.2019 |
| Volume | 318 |
| Issue | 1 |
| Year Published | 2019 |
| Language | English |
| Publisher | American Physiological Society |
| Contributing office(s) | Leetown Science Center |
| Description | 13 p. |
| First page | R17 |
| Last page | R29 |