In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
Archives of Virology
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Abstract
Suggested Citation
Carossino, M., Ip, H., Richt, J.A., Schultz, K., Harper, K., Loynachan, A.T., Del Piero, F., Balasuriya, U.B., 2020, Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques: Archives of Virology, v. 165, p. 2373-2377, https://doi.org/10.1007/s00705-020-04737-w.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques |
| Series title | Archives of Virology |
| DOI | 10.1007/s00705-020-04737-w |
| Volume | 165 |
| Publication Date | August 05, 2020 |
| Year Published | 2020 |
| Contributing office(s) | National Wildlife Health Center |
| Description | 5 p. |
| First page | 2373 |
| Last page | 2377 |