Release of hatchery-reared juvenile cisco (Coregonus artedi) is an important tool for recovering Great Lakes populations, but post-release survival is unknown. Telemetry using small acoustic tags provides opportunities to assess the efficacy of hatchery-reared fish releases. However, better understanding of the tolerance of juvenile cisco to acoustic tags is needed. Juvenile cisco mortality and tag retention as a function of tag size:body size (Relative Tag Weight, RTW) was observed for 30 d post tag implantation in the laboratory. Tag loss and mortality increased with RTW. A single mortality occurred for RTW ≤ 3% and tag retention and survival was <50% for RTW > 7.0%. Logistic regression predicted >95% survival and tag retention to 30 d for RTW < 2.5%, with full survival of study fish for RTW of ≤2%. Sedation and surgery times did not affect survival of tagged fish, but results of anesthesia-only and sham surgeries highlight the need to minimize handling effects for effective acoustic telemetry studies. Our findings clarify thresholds of RTW and indicate that juvenile cisco can receive acoustic tag implants. Observing these limitations can improve the effectiveness of acoustic telemetry to assess success of cultured juvenile fish releases for conservation or restoration of native forage fish populations. Evaluation of the effects of acoustic tags over longer time periods and under environmental conditions, like those at release sites, are needed to further validate this technology.