Using enclosed Y-mazes to assess chemosensory behavior in reptiles
Journal of Visualized Experiments
Links
- More information: Publisher Index Page (via DOI)
- Open Access Version: Publisher Index Page
- Download citation as: RIS | Dublin Core
Abstract
Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and present focal animals with a binary choice. In our Y-maze studies, a scenting animal is restricted to one arm of the maze to leave a scent trail and is removed once scent-laying parameters have been met. Then, depending on the trial type, either the focal animal is allowed into the maze, or a competing scent trail is created. The result is a record of the focal animal's choice and behavior while discriminating between the chemical cues presented. Here, two Y-maze apparatuses tailored to different invasive reptile species: Argentine black and white tegu lizards (Salvator merianae) and Burmese pythons (Python bivittatus) are described, outlining the operation and cleaning of these Y-mazes. Further, the variety of data produced, experimental drawbacks and solutions, and suggested data analysis frameworks have been summarized.
Suggested Citation
Parker, M.R., Currylow, A.F., Tillman, E.A., Robinson, C.J., Josimovich, J.M., Bukovich, I.M., Nazarian, L.A., Nafus, M.G., Kluever, B.M., Yackel Adams, A.A., 2021, Using enclosed Y-mazes to assess chemosensory behavior in reptiles: Journal of Visualized Experiments, v. 170, e61858, 19 p., https://doi.org/10.3791/61858.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Using enclosed Y-mazes to assess chemosensory behavior in reptiles |
| Series title | Journal of Visualized Experiments |
| DOI | 10.3791/61858 |
| Volume | 170 |
| Publication Date | April 07, 2021 |
| Year Published | 2021 |
| Language | English |
| Publisher | Jove |
| Contributing office(s) | Fort Collins Science Center |
| Description | e61858, 19 p. |
| Online Only (Y/N) | Y |