Pollen assemblages from five mid-Pliocene, deep-marine and shallow-marine sites in the North Atlantic Ocean region were analyzed quantitatively to estimate vegetational composition and terrestrial paleoclimates during a time interval centered at about 3 Ma. Three subarctic sites (58–67°N latitude) from the Norwegian Sea, northern Iceland, and the Labrador Sea yielded pollen assemblages characteristic of mixed conifer-hardwood vegetation; these assemblages have no close modern analogs, but the most similar modern vegetation is that of the Tsuga (hemlock)-northern hardwood forests of Newfoundland and Nova Scotia (45–50°N). The presence of such vegetation at these sites indicates mid-Pliocene January temperatures from 4 to 10°C warmer than today and a northward shift of the deciduous forest zone into areas presently occupied by boreal forests. Pollen assemblages from the mid-latitude (36°N) Yorktown Formation site in southeastern Virginia, USA represent Quercus-Carya-Pinus (oak-hickory-pine) forests, similar to those presently occupying much of the Atlantic coastal plain of the United States. Modern analogs for this site are located off the coasts of North Carolina and South Carolina, indicating mid-Pliocene January temperatures 3–5°C warmer than today. In southwestern Florida (27°N), pollen assemblages from the mid-Pliocene Pincrest Beds are representative of pine-dominated communities, similar to Pinus palustris/P. ellittii forests (longleaf/slash pine) growing in Florida today. Close modern analogs for the Pinecrest Beds are located at numerous sites around the Florida peninsula, indicating mid-Pliocene temperatures about the same as today for this region.

These sites show a gradient from much higher than present temperatures at high latitudes to little change from present temperatures at low latitudes and low elevations; a similar pattern is shown by mid-Pliocene sea-surface and isotopic data. Such a gradient also is consistent with the northward migration of the boreal forest zone, covering sites as far north as Meighen and Banks Islands, a northward shift of the deciduous-boreal forest boundary in both North America and Europe, and little change in vegetational distribution in low elevation sites in subtropical latitudes during the mid-Pliocene warm interval. Atmospheric temperaturs estimated from vegetational distributions indicate a Pliocene to modern temperature anomaly of a similar pattern and magnitude to that indicated by sea-surface temperatures. These data are most consistent with current general circulation model simulations of climate changes in response to increased meridional heat transport.