Python bivittatus is established in the Greater Everglades Ecosystem in southern Florida, USA. Documented predators on pythons in Florida include Alligator mississippiensis (American Alligator; Snow et al. 2006. Herpetol. Rev. 37:81–81), Drymarchon couperi (Gulf Coast Indigo Snake; Andreadis et al. 2018. Herpetol. Rev. 49:341–342), Lynx rufus (Bobcat; McCollister et al. 2021. Southeast. Nat. 20:N55–N59), and possibly Ursus americanus floridanus (Florida Black Bear; McCollister et al. 2021. op cit.). We documented mortality events of hatchling and juvenile P. bivittatus while conducting radiotelemetry studies in the Greater Everglades Ecosystem.