The river continuum concept predicts a longitudinal gradient in the structure and functioning of rivers. Impoundments potentially change this continuum by reorganizing nutrient transport and storage in the system. To determine if predictions made by the river continuum concept relative to fish assemblage trophic structure hold for a temperate river transformed into a reservoir cascade, we examined longitudinal trends in the distribution of biomass among feeding guilds over 30 reservoirs of the Tennessee River (USA). Fish assemblages were sampled over a 12-year period, and fishes were classified as detritivores, herbivores, invertivores, piscivores, or planktivores. Biomass of all feeding guilds increased with catchment area (i.e., in a downstream direction). However, representation of herbivores and planktivores within the fish assemblage, as indexed by percent biomass composition, increased with catchment area, whereas representation of detritivores, invertivores, and piscivores decreased. In general, the predictions made by the river continuum concept apply to the Tennessee River reservoir cascade despite the major environmental transformation caused by the series of impoundments. However, transformation of lotic into lentic systems promotes autochthonous primary production and proliferation of herbivores and planktivores that reduces relative representation of other guilds. The marked longitudinal pattern in feeding guild structure in the reservoir cascade reflected changes in production sources following impoundment of the river.