Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses

Scientific Reports
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Abstract

Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal swabs, and ocular fluid were collected from white-tailed deer (Odocoileus virginianus). Tissue samples and nasal swabs acted as a control to compare extraction and DNA suitability for microsatellite analysis for nasal swabs and ocular fluid extracted from FTA Elute cards. We determined that FTA Elute cards improved the extraction time and storage of samples and that nasal swabs and ocular fluid containing pigmented fluid were reasonable alternatives to traditional tissue DNA extractions.

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Publication type Article
Publication Subtype Journal Article
Title Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses
Series title Scientific Reports
DOI 10.1038/s41598-021-89390-2
Volume 11
Year Published 2021
Language English
Publisher Nature Publications
Contributing office(s) Coop Res Unit Leetown
Description 10003, 7 p.
Country United States
State Pennsylvania
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