Some northern Wisconsin lakes have shown declines in catches of age-0 Walleye Sander vitreus in standardized fall electrofishing sampling, suggesting that recruitment bottlenecks are occurring in the first several months of life. In 2016 and 2017, we sampled six lakes with declining trends in natural Walleye recruitment (D-NR lakes) and seven lakes with a history of sustained natural recruitment (S-NR lakes) to determine if timing of potential recruitment bottlenecks for age-0 Walleye were consistent among D-NR lakes and if abiotic and biotic metrics differed between D-NR and S-NR lakes. We also examined diets of larval Walleye to assess prey items that may be important to early growth and survival and to determine if occurrence of piscivory was related to larval Walleye total length. Differential patterns in the presence and absence of age-0 Walleye at different life history stages in the first 6 months of life suggested that recruitment bottlenecks in D-NR lakes were consistently occurring before mid-July (five of six lakes). Mean Secchi depth, surface conductivity, abundance of larval Yellow Perch Perca flavescens, and most metrics of zooplankton abundance and mean size were similar between D-NR and S-NR lakes. Log₁₀ transformed number of adult Walleye per hectare was lower and adult mean total length was higher in D-NR lakes. Across all lakes, diets of larval Walleye consisted of zooplankton and larval fish and the occurrence of piscivory was higher than reported in previous studies and was positively related to total length of larval Walleye. Causes of recruitment bottlenecks in D-NR lakes remain unclear, making it difficult to identify management actions that might be implemented to circumvent these bottlenecks. However, our results indicate that future research should focus on the period between egg deposition and mid-July.