The quantitative determination of chlorophylls A and B from freshwater algae without interference from degradation products
Journal of Research of the U.S. Geological Survey
By: W. Thomas Shoaf and Bruce W. Lium
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Abstract
Chlorophyll was efficiently extracted from freshwater algae with dimethyl sulfoxide. The extract was chromatographed on commercially available thin-layer cellulose sheets, 2 percent methanol and 98 percent petroleum ether being used as the solvent. Chlorophylls a and b, and phaeophytins a and b were cleanly separated from each other and other degradation products. No artifacts were formed. Recoveries of chlorophylls a and b were 98 and 96 percent as measured by spectrophotometry or fluorometry.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | The quantitative determination of chlorophylls A and B from freshwater algae without interference from degradation products |
| Series title | Journal of Research of the U.S. Geological Survey |
| Volume | 5 |
| Issue | 2 |
| Year Published | 1977 |
| Language | English |
| Publisher | U. S. Geological Survey |
| Description | 2 p. |
| First page | 263 |
| Last page | 264 |