Potential sources of suspended sediment and sediment-bound phosphorus (sedP) were studied in the Kinnickinnic River (51 square kilometers), a heavily urbanized tributary to Lake Michigan (90% urban land use) in Milwaukee, Wisconsin. The river is 60% concrete lined channels, with few unlined reaches. From September 2019 through August 2020, an integrated study of sediment budget and sediment fingerprinting was conducted to quantify upland and stream corridor sources of suspended sediment and sedP using Sediment Source Assessment Tool (SedSAT) methods with a suite of trace elements. Passive suspended sediment samplers were installed at three sites. Soft, fine-grained streambed sediment was collected at 10 rapid geomorphic assessment (RGA) sites. An inventory of bank erosion and soft-sediment deposition was done at each of the 18 RGA sites, which were selected to represent a range of stream sizes and geomorphic conditions. Sources of suspended sediment varied with streamflow; the primary source was from roadways in residential areas followed by eroding streambanks. Industrial/commercial areas contributed 1% of the suspended sediment at the streamgage during the study period, whereas green space contributed 18% of the suspended sediment at a mid-basin monitoring location downstream of an unlined reach. The dominant sources of streambed sediment, like suspended sediment, throughout the basin were eroding banks and residential areas, with green space and industrial/commercial area signatures present locally. In contrast with previous studies in agricultural and mixed-use basins, the urban Kinnickinnic River had limited storage of soft sediment, due to the hydrologically flashy system and concrete lined channels. However, eroding streambank sources contribute 50% of the streambed sediment, but only 9% of suspended sediment within this tributary to the Great Lakes.