Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
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Abstract
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction Reagent. RHDV2 was detected in all nine samples. Cycle threshold values were higher in the RT-PCR following SwiftX extraction (mean: 3.79), indicating that the second extraction method resulted in approximately a 1 log10 reduction in sensitivity. A second freeze–thaw for the samples and less tissue extracted using SwiftX may have contributed additionally to the loss in sensitivity.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples |
| Series title | BioTechniques |
| DOI | 10.2144/btn-2022-0022 |
| Volume | 74 |
| Issue | 4 |
| Year Published | 2023 |
| Language | English |
| Publisher | Eaton Publishing Company |
| Contributing office(s) | National Wildlife Health Center |
| Description | 2 p. |
| First page | 156 |
| Last page | 157 |
| Google Analytic Metrics | Metrics page |