Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
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Abstract
We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction Reagent. RHDV2 was detected in all nine samples. Cycle threshold values were higher in the RT-PCR following SwiftX extraction (mean: 3.79), indicating that the second extraction method resulted in approximately a 1 log10 reduction in sensitivity. A second freeze–thaw for the samples and less tissue extracted using SwiftX may have contributed additionally to the loss in sensitivity.
Suggested Citation
Hofmeister, E.K., Griffin, K., and Ip, H., 2023, Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples: BioTechniques, v. 74, no. 4, p. 156-157, https://doi.org/10.2144/btn-2022-0022.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples |
| Series title | BioTechniques |
| DOI | 10.2144/btn-2022-0022 |
| Volume | 74 |
| Issue | 4 |
| Publication Date | May 10, 2023 |
| Year Published | 2023 |
| Language | English |
| Publisher | Eaton Publishing Company |
| Contributing office(s) | National Wildlife Health Center |
| Description | 2 p. |
| First page | 156 |
| Last page | 157 |