The Earth Observing-1 (EO-1) Hyperion instrument provides 220 spectral bands with wavelengths between 400 and 2500 nm at 30 m spatial resolution, which covers a 7.5 km by 100 km area on the ground. The EO-1 spacecraft has another multispectral sensor called the Advanced Land Imager (ALI), which has 10 spectral bands with wavelengths between 400 and 2350 nm at 30 m spatial resolution. The Moderate Resolution Imaging Spectroradiometer (MODIS) sensor onboard the Terra spacecraft was launched in Dec., 1999, and flies approximately 30 minutes behind EO-1. Nearsimultaneous observations from Terra MODIS, EO-1 ALI and Hyperion over a well characterized Railroad Valley Playa in Nevada (RVPN) target are chosen for this study. A uniform region of interest (ROI) within the playa within latitudes and longitudes of 38.48 and -115.71 to 38.53 and -115.66 was chosen for this analysis. A representation of the ground spectra during every near-simultaneous acquisition of MODIS and ALI is obtained using EO-1 Hyperion data. Using the EO-1 Hyperion derived top-of-atmosphere (TOA) reflectance profile along with the ALI and MODIS relative spectral responses (RSR), simulated reflectance for the matching band pairs is calculated. The Hyperion simulated TOA reflectance results are compared to the measured TOA reflectance trends of ALI and MODIS. The long-term measured versus simulated reflectance results are used to examine the relationships and calibration differences between the ALI and MODIS sensors.