Widely separated basalt lava-flow outcrops in north-central Oregon, USA, expose products of a single eruptive episode. A Pliocene lava flow, here informally termed the Tetherow basalt, issued from vents near Redmond, in the Deschutes basin of Oregon, as a plains-forming basalt now exposed in continuous outcrops northward for 60 km. A similar basalt crops out 47 km farther north, near Maupin, within what was then a slightly incised ancestral Deschutes River canyon. The northernmost outcrops of this lava flow lie on Fulton Ridge, in the Dalles basin, near the confluence of the Deschutes and Columbia Rivers. Complementary lines of evidence confirm these rocks are all from the same volcanic eruption. Outcrops in the Deschutes and Dalles basins are chemically similar high-titanium basalts, petrographically similar to each other and distinct from other lava flows in the area. Paleomagnetic directions from 11 scattered sites are similar and indistinguishable by various tests for a common mean. Three new ⁴⁰Ar/³⁹Ar ages indicate the Tetherow basalt eruption occurred between 5.5 Ma and 5.0 Ma, likely at ca. 5.2 Ma. The widely separated outcrops of this lava flow span 160–180 km along the ancestral Deschutes River and downstream Columbia River. The lava flow’s length and erupted volume of 15–20 km³ are extraordinarily large in a non-flood-basalt setting. This lava flow provides a datum with which to describe regional physiographic history, assess incision rates, and infer tectonic history. Spanning different depositional basins, the Tetherow basalt is a useful chronologic and stratigraphic marker bed.