The lesser prairie-chicken (Tympanuchus pallidicinctus) is a species of conservation concern on the Southern High Plains of Texas and New Mexico, USA. Because fragmentation and isolation have increased since pre-settlement, dispersal through this heterogeneous landscape may be constrained, with serious implications for conservation and management of this species. Our objectives were to quantify landscape connectivity for lesser prairie-chickens within a patch network of potentially isolated leks (breeding display grounds), and examine effects of land use change on modeled lesser prairie-chicken movements through the landscape. We used graph theory to quantify structural landscape connectivity and circuit theory to quantify functional landscape connectivity for lesser prairie-chickens in the Sand Shinnery Oak Prairie Ecoregion of the Southern High Plains. There was a high degree of clustering among leks (n = 1,023 leks), with a 41.9-km coalescence distance of the network. We identified 3 leks as cutpoints within the network, meaning if the habitat patches containing these leks were fragmented, the remaining leks would become isolated from each other. We also identified several leks that were important for maintaining overall population connectivity for lesser prairie-chickens on the Southern High Plains. Conservation Reserve Program land was important for maintaining connectivity among leks to the north and west of the main lek core area located in New Mexico, but wind energy development constrained pathways to the north and south of this main lek core area. Our results suggest that landscape connectivity was reduced by row-crop agriculture and energy production and facilitated by the Conservation Reserve Program within the Sand Shinnery Oak Prairie Ecoregion.