An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus)

Conservation Genetics Resources
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Abstract

Sauger (Sander canadensis) and walleye (Sander vitreus) are closely related North American fish species that are often managed by fishery agencies throughout their ranges. However, genotyping resources for sauger are presently limited to a small set of microsatellite loci. We evaluated whether primers in an existing walleye genotyping-in-thousands panel could amplify single nucleotide polymorphism loci (SNPs) in sauger. We identified 71 primer pairs that amplify 118 SNPs in both species. Allele frequency differences were large enough to confidently distinguish the species and identify hybrids. Additionally, we identified 41 loci with observed heterozygosity > 0.1 in sauger; these markers may be useful for simple population genetic analyses and parentage analysis when few contributors are present and for differentiating highly structured populations.

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Publication type Article
Publication Subtype Journal Article
Title An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus)
Series title Conservation Genetics Resources
DOI 10.1007/s12686-023-01329-z
Volume 16
Year Published 2023
Language English
Publisher Springer Link
Contributing office(s) Coop Res Unit Leetown
Description 8 p.
First page 103
Last page 110
Country United States
State Minnesota, Wisconsin
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