The U.S. government advances One Health coordination through the best practices of jointly developing shared priorities and utilizing formalized coordination platforms to connect partners from public health, agriculture, wildlife, environment, and other sectors at the national, subnational (e.g. state, tribal, local, and territorial), and non-governmental levels (e.g. academia, industry, non-governmental organizations, and the public) levels. Coordinated efforts were strengthened through the U.S. One Health Zoonotic Disease Prioritization (OHZDP) workshop in 2017, which led to the prioritization of eight zoonotic diseases and development of next steps and action plans for One Health collaboration across federal agencies (Prioritizing Zoonotic Diseases for Multisectoral, One Health Collaboration in the United States, 2017). This One Health best practice was used to prioritize the top endemic and emerging zoonoses of greatest concern for the United States, identify gaps, and define plans for U.S. government One Health collaboration to address the priority zoonotic diseases. Multiple actions that strengthened One Health coordination were enacted, including establishing the One Health Federal Interagency COVID-19 Coordination (OH-FICC) group. To further advance One Health in the United States, Congress mandated that the U.S. Centers for Disease Control and Prevention, the Department of the Interior, and the U.S. Department of Agriculture collaborate to develop the National One Health Framework to Address Zoonotic Diseases and Advance Public Health Preparedness in the United States as well as formalize a One Health, multisectoral coordination mechanism, the United States One Health Coordination Unit at the federal level (Appropriations Committee Report, 2021; Consolidated Appropriations Act, 2023). Enhancing national level One Health coordination in the United States has also helped advance collaboration with subnational and non-governmental levels to address timely One Health issues.