Continental rejuvenation results from the tectonic reactivation of crustal structures and lithospheric reworking by mantle flow. Geochemical observations and field mapping have traditionally provided the primary evidence for the secular evolution of crustal composition and tectonic processes during continental rejuvenation. Nonetheless, the impact of continental rejuvenation on the observed present-day strain rate and orogenic-scale lithospheric structure has not been well constrained. The pre-existing E-W–trending Central China Orogenic Belt has been overprinted by the N-S–trending Central Longitudinal Seismic Belt and constitutes the intracontinental West Qinling Syntaxis in central China, where the tectonic setting changes eastward from contraction to extension. Combining updated global positioning system data and high-resolution crustal seismic tomography, we reveal a modern continental rejuvenation process within the West Qinling Syntaxis in central China. The northward extrusion of the Tibetan Plateau's weak lithospheric layer (middle-lower crust and lithospheric mantle) of southwestern China relative to the rigid Sichuan Basin/Ordos Block of the eastern West Qinling Syntaxis results in regional dextral shearing that shapes the Central Longitudinal Seismic Belt and defines the eastern Tibetan Plateau margin. The pre-existing E-W–trending Central China Orogenic Belt has been preserved above the brittle-ductile transition zone, and the northward movement of the deep lithospheric layer drives the deformation of the upper crust in the West Qinling Syntaxis. Our results, along with previous studies, suggest the presence of an intracontinental lithospheric interchange structure in central China. The continental rejuvenation of the West Qinling Syntaxis results from a combination of fault reactivation in the upper crust (Stage I, Eocene–Oligocene) and reworking of the deep lithosphere (Stage II, middle–late Miocene) related to the plateau-wide shift in stress accommodation ultimately driven by the redistribution of mass outward from the central Tibetan Plateau. At present, the transition zone between the high- and low-velocity anomalies along the Central Longitudinal Seismic Belt not only shapes the landscape boundary but controls the size and recurrence interval of earthquakes within the West Qinling Syntaxis in central China.